Wednesday, March 29, 2017
University of Hawaii at Manoa
University of Hawaii System
College of Tropical Agriculture and Human Resources
 
Diagnosis. Here is an outline presented as an overview of wilt diagnosis for ginger. More information is included on the linked pages below.

1. Signs
  • bacterial ooze from infected tissues
  • bacterial streaming from infected tissues
2. Symptoms

3. Serological tests
  • ELISA
  • Immunostrips (Immunostrip user guide: https://orders.agdia.com/Documents/m182.pdf)
  • PCR enrichment. Growers can bring in their soils for analysis of bacterial wilt of ginger at the Komohana CES office; this service is NOT offered
    at other ADSC locations.  Analysis D1 at $12.00 per sample.
4. DNA tests
5. Bioassay

6. Testing services
Comparison of  various testing methods for diagnosis of bacterial wilt of edible ginger:
Diagnostic
method
Time
required 
 Reliability  Cost 
 Signs  Minutes  High  Low
 Symptoms  Minutes  Low  Low
 Serological tests  Minutes  High  Moderate
 DNA tests  Hours  High  High (equipment, skill)
 Bioassay  Days  Moderate  Moderate (pots, medium, 
 Testing services  Days  High  Moderate


Pathogen detection. The bacterial wilt pathogen can be detected using a number of different laboratory methods and from several different sources:

1. Soil
2. Water
3. Infected plant tissue




Video: Detection of Ralstonia solanacearum race 4 using PCR (Polymerase Chain Reaction)
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